Cold-Adapted Reassortants of Influenza A Virus in MDCK Cells
نویسندگان
چکیده
منابع مشابه
Development and Sequence Analysis of a Cold-Adapted Strain of Influenza A/New Caledonia/20/1999(H1N1) Virus
Background and Aims: Vaccination is the most effective method to prevent influenza infection. Among the available vaccines, cold-adapted live-virus vaccines are suitable approach that have been produced and evaluated for recent years in few countries. The goal of this project was to derivate a cold adapted variant of the influenza A/New Caledonia/20/1999(H1N1). Materials and Methods: Influenza ...
متن کاملCold-adapted recombinants of influenza A virus in MDCK cells. I. Development and characterization of A/Ann Arbor/6/60 X A/Alaska/6/77 recombinant viruses.
Recombinant influenza viruses made at 25 and 33” in Madin-Darby canine kidney (MDCK) cells using the cold-adapted A/Ann Arbor/6/60 virus and the wild-type A/ Alaska/6/‘77 virus were biologically and genetically analyzed. Eight recombinants were separated into two phenotypic groups based on cold-adapted (ca) and temperature-sensitive (ts) markers: ca and ts, ca and non-m. The ca recombinants sho...
متن کاملCold-adapted variants of influenza virus A. I. Comparison of the genetic properties of ts mutants and five cold-adapted variants of influenza virus A.
The genetic properties of seven cold-adapted variants of influenza virus A were compared with those of nine Lfluorouracil (5-FU)-induced ts mutants. The 5-FU mutants had previously been placed into seven complementation-recombination groups; five of the seven cold-adapted variants also had the ts phenotype, and all five were shown to share the group 1 lesion. Three of the cold variants also had...
متن کاملDevelopment of an Influenza A Master Virus for Generating High-Growth Reassortants for A/Anhui/1/2013(H7N9) Vaccine Production in Qualified MDCK Cells
In 2013, the first case of human infection with an avian influenza A virus (H7N9) was reported in China, and the human infection with this virus has continued as of 2016. At the request of the WHO, we have successfully developed candidate reassortant vaccine virus using A/Anhui/1/2013 and the high egg-growth master virus A/PR/8/1934. Recent plans regarding influenza vaccine production include u...
متن کاملdevelopment and sequence analysis of a cold-adapted strain of influenza a/new caledonia/20/1999(h1n1) virus
background and aims: vaccination is the most effective method to prevent influenza infection. among the available vaccines, cold-adapted live-virus vaccines are suitable approach that have been produced and evaluated for recent years in few countries. the goal of this project was to derivate a cold adapted variant of the influenza a/new caledonia/20/1999(h1n1). materials and methods: influenza ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1983
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1983.tb03574.x